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ROSENOW'S "RECIPES" FOR: COLDS, INFLUENZA: VACCINE PREPARATION DETAILS [34R3, p. 408]
Rosenow, E.C., Cataphoresis as a control of specificity of streptococcal vaccines. Influenzal streptococcus vaccine in the prevention and treatment of infections of the respiratory tract. Jour. Immunol. 26:401-433 (May) 1934:

VACCINE PREPARATION DETAILS (COLDS, INFLUENZA) 34R3, p. 408
"In this study the vaccines used for prophylactic and active immunization against colds and influenza were prepared from the respective streptococci by adding enough of the dense suspension in glycerin-salt solution to 0.85 per cent sodium chloride solution to bring the density to that of the dextrose-brain broth culture, or approximately 2 billion streptococci per cc. The suspensions were heated to 75 degrees C for one hour, in vials of a capacity of 30cc., sealed with perforated screw-caps containing a rubber disk; the vials were completely immersed in water. For prophylactic inoculation, 0.3, 0.5 and 1.0 cc. were injected subcutaneously, a week apart, and then one injection of 1.0 cc was given once a month throughout the season when colds and influenza are prevalent. The dosage for children was reduced according to age. If the reactions proved too severe for hypersensitive patients the dosage was reduced according to the degree of reaction, but this was almost never necessary. For treatment, regardless of the duration or character of the respiratory manifestation, 0.3 cc of the vaccine was given subcutaneously as the initial injection; this was followed in 24 hours by 0.5 cc, and a day or 2 later by 1.0 cc subcutaneously, provided the reactions were not too severe and the patient's condition was improved or was no worse. In order to facilitate absorption as much as possible, the area of subcutaneous injection was massaged immediately, and the patient was instructed to massage it once or twice daily for three or four days following injection ... regardless of the degree of local reaction." [34R3, p. 408]

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