DEXTROSE-BRAIN BROTH MEDIUM  34R3


  --"...cultures made by usual methods ... may yield the easily


cultivable saprophytic organisms, including streptococci, instead


of the highly sensitive, disease-producing strains. ... Special


mediums, such as dextrose-brain broth and dextrose-brain agar,


which afford reduced oxygen tension and other favorable conditions


for growth, are essential in the primary culture in many diseases


to insure isolation of the really causative streptococci." [34R3]






STREPTOCOCCUS V.S. VIRUS IN COMMON COLD AND INFLUENZA  34R3-403


  "It is becoming more generally recognized, despite the


demonstrated importance of the 'virus' factor, especially in colds,


that the more serious manifestations referable to the respiratory


tract and remote tissues, incident to epidemic colds and influenza,


are due to visible, cultivable organisms, chiefly streptococci, and


more rarely to Bacillus influenzae and staphylococci.  The results


of studies on cataphoresis and virulence [and with vaccines


indicate] ... that these streptococci are more often of primary


than of secondary importance." [34R3, p. 403]






VACCINE PREPARATION DETAILS, ETC.  34R3, p. 408


  "In this study the vaccines used for prophylactic and active


immunization against colds and influenza were prepared from the


respective streptococci by adding enough of the dense suspension in


glycerin-salt solution to 0.85 per cent sodium chloride solution to


bring the density to that of the dextrose-brain broth culture, or


approximately 2 billion streptococci per cc.  The suspensions were


heated to 75 degrees C for one hour, in vials of a capacity of


30cc., sealed with perforated screw-caps containing a rubber disk;


the vials were completely immersed in water.  For prophylactic


inoculation, 0.3, 0.5 and 1.0 cc. were injected subcutaneously, a


week apart, and then one injection of 1.0 cc was given once a month


throughout the season when colds and influenza are prevalent.  The


dosage for children was reduced according to age.  If the reactions


proved too severe for hypersensitive patients the dosage was


reduced according to the degree of reaction, but this was almost


never necessary.  For treatment, regardless of the duration or


character of the respiratory manifestation, 0.3 cc of the vaccine


was given subcutaneously as the initial injection; this was


followed in 24 hours by 0.5 cc, and a day or 2 later by 1.0 cc


subcutaneously, provided the reactions were not too severe and the


patient's condition was improved or was no worse.  In order to


facilitate absorption as much as possible, the area of subcutaneous


injection was massaged immediately, and the patient was instructed


to massage it once or twice daily for three or four days following


injection ... regardless of the degree of local reaction." [34R3,


p. 408]






SYMPTOMLESS FOCI, IMPORTANCE OF - TAKE II [34R5-721]


"Lucas, in an extended study, reported that 319 of 364 patients


having symptoms of various diseases, in many of whom inadequate


consideration of foci had previously been given, were relieved from


symptoms after thorough eradication of foci of infection in the


dental area.  Streptococci were isolated from nearly all of these


foci, which included the remnants of the enamel organ of unerupted


teeth.  Vaccines, even though specific, have only limited value


when given to patients without removal of evident foci."[721] 


[Lucas, C.D., "Periapical Infection", Dental Cosmos 71: 555-564,


June 1929].